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Understanding Molecular Biology

In this free online course, learn about the processes and techniques involved in Understanding Molecular Biology.

Publisher: NPTEL
In this free online course, learn about the techniques and methods involved in Understanding Molecular Biology. Learn the definition of sequencing, blotting and polymerase chain reaction techniques. The Northern Blotting technique, which is used to detect the RNA that is present in the cell will be discussed. Boost your Understanding of Molecular Biology skills by studying this comprehensive course.
Understanding Molecular Biology
  • Duration

    6-10 Hours
  • Students

  • Accreditation






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In this free online course, you are introduced to the world of Understanding Molecular Biology. You will explore the fact that the Polymerase Chain Reaction technique is used to amplify many double standard DNA molecules that have the same size and sequence using an enzymatic method and cycling condition. DNA is a nucleic acid that is composed of two complementary nucleotide building blockchains. These nucleotides are made up of a phosphate group, five-carbon sugars, and a nitrogen base. You will be introduced to the concept of DNA replication, which is a process of duplication of the entire genome before the division of cells, and the extreme accuracy of DNA replication is necessary to preserve the integrity of the genome in successive generations. You will learn that the four nitrogen bases that are found in DNA, are linked in a repeated pattern by hydrogen bonding between the nitrogen bases and that the linking of the two complementary strands is called hybridization. Demonstrations, problems, examples and assessment questions in this instructor-led video-based course, are designed to provide you with a comprehensive foundation, which will enable you to take serious steps into this career.

The course begins by introducing you to the definition of DNA replication characteristics and Polymerase Chain Reaction Setup. Then, the fact that primer secondary structures arise as a result of intra or intermolecular attraction within the primer, which eventually reduces the yield of amplification as the availability of single-stranded primers are limited for the PCR, will be discussed. You will explore the thermal cycler, which is an instrument that carries out the amplification of the DNA using the polymerase chain reaction. In addition to this, the fact that the PCR is a repeated cycle reaction that involves a mechanism of DNA replication and results in the production of multiple copies of DNA from a single one, will be debated. It will be clarified that Primers should be designed in such a way that there should be no homology within the template other than the target site. This will result in non-specific binding and amplification. You will recognize that the advantages of using the Polymerase Chain Reaction technique are speed, ease of use, sensitivity and robustness when compared to other techniques used in clinical diagnostic tests.

Next, you will learn that the PCR technology has become the basis for a broad spectrum of clinical diagnostic tests for various infectious agents, including viruses and bacteria and that it helps in detecting the presence of infectious diseases in the donated blood samples. You will gain insight into the fact that a primer is a short DNA stretch that serves as a starting point for DNA synthesis. You will then determine that the appearance of a smear that has visible bands, indicates that the genomic DNA has been completely digested and the suitability of the sample for southern blotting. You will be taught that Northern Blotting technique is used to detect the RNA that is present in the cell. You will learn that the two examples of methods that are used in DNA Sequencing are Sanger’s Sequencing Method and Maxam Gilbert Method. Finally, you will study Polymerase Chain Reaction techniques, sequencing and blotting techniques, DNA amplification, and PCR in the tissue culture. This course will be of interest to Biotechnology students, or those professionals in Experimental Biotechnology. Why wait? Start this course today and become an expert in Molecular Biology.

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