DNA Delivery and Protein Synthesis | Free Course | Alison
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DNA Delivery and Protein Synthesis

Learn the strategies and approaches involved in DNA delivery and protein production from this free online course.

Biology
Free Course
In this free online course, you will be provided with a comprehensive knowledge of transformation and electroporation in bacterial cells. You will be introduced to DNA delivery in host cells and the procedure for screening recombinant clones. Learn about protein synthesis machinery along with host as production machinery. By the end of the course, you will be familiar with E.coli, yeast, insect cell line and mammalian cells as expression systems.
  • Duration

    3-4 Hours
  • Assessment

    Yes
  • Certification

    Yes
  • Responsive

    Yes
  • Publisher

    NPTEL

Description

Modules

Outcome

Certification

View course modules

Description

DNA Delivery and Protein Synthesis is a free online course that begins with an introduction to the different methods of entry of a recombinant DNA into the host species. You will learn about competent cells, the discovery of transformation, and the mechanism of transformation. You will be able to explain the concept of electroporation. Several strategies utilized to deliver the recombinant DNA into the mammalian expression system or mammalian cells will be made clear. Therefore, you will learn about the advantages of these approaches.

The course then explains the importance of screening recombinant clones as well as the approaches to screen the molecules. You will learn about antibiotic sensitivity, antibiotic resistance genes, and the confirmation of cloned DNA. Next, you will analyze the processes involved in insertional inactivation, blue-white screening, and complementation of mutation.

Furthermore, you will be exposed to the different steps which are involved in a typical protein production machinery. You will learn about the factors to be considered before choosing a host expression vector system. Finally, you will learn about the mammalian expression system alongside protein synthesis in yeast and insect cell lines. This free online course will be of interest to students, researchers, and anyone with an interest in the field of genetics. So, register for this course and start your next learning journey today.

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Learning Outcomes

By the end of this course, you will be able to:


  • Discuss DNA delivery in host cells
  • Explain the concept and discovery of transformation
  • Explain the preparation of competent cells
  • Discuss the delivery of DNA in mammalian cells
  • Discuss antibiotic sensitivity
  • Explain the steps involved in the synthesis of protein
  • Discuss the criteria for selecting an expression system
  • Discuss the components of an e.coli expression system as well as their functions
  • Analyze yeast as an expression system
  • Explain protein production in insect cell line
  • Discuss the complexities of the mammalian expression system

Certification

All Alison courses are free to enrol, study and complete. To successfully complete this Certificate course and become an Alison Graduate, you need to achieve 80% or higher in each course assessment. Once you have completed this Certificate course, you have the option to acquire an official Certificate, which is a great way to share your achievement with the world. Your Alison Certificate is:

Ideal for sharing with potential employers - include it in your CV, professional social media profiles and job applications
An indication of your commitment to continuously learn, upskill and achieve high results
An incentive for you to continue empowering yourself through lifelong learning

Alison offers 3 types of Certificates for completed Certificate courses:

Digital Certificate - a downloadable Certificate in PDF format, immediately available to you when you complete your purchase
Certificate - a physical version of your officially branded and security-marked Certificate, posted to you with FREE shipping
Framed Certificate - a physical version of your officially branded and security-marked Certificate in a stylish frame, posted to you with FREE shipping

All Certificates are available to purchase through the Alison Shop. For more information on purchasing Alison Certificates, please visit our FAQs. If you decide not to purchase your Alison Certificate, you can still demonstrate your achievement by sharing your Learner Record or Learner Achievement Verification, both of which are accessible from your Dashboard. For more details on our Certificate pricing, please visit our Pricing Page.

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