Biotechnology - Cell Biology Techniques
Learn cell structures and functions and the various cell biology techniques in biotechnology in this free online course.Publisher: NPTEL
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The advent of microscopes in the 17th century played a massive role in our understanding of cells and this free online course comprehensively studies various cell biology techniques, especially as it applies to biotechnology. This course will introduce you to prokaryotic and eukaryotic cells and the different applications of these types of cells in scientific experiments. Gain insight into the fact that all these cells are required to propagate before they can be used in scientific experiments and this is done through the use of different growth mediums. You will explore the common media constituents for bacterial growth, their composition and applications. This knowledge gets applied when you are preparing the microbiology medium and the relevant precautions you need to take while carrying out these experiments. You will be introduced to the concept of differential centrifugation, which is based on the differences in sedimentation rates of the biological properties of different sizes, shapes, and densities.
The course starts by introducing you to the fractionation of prokaryotic as well as the eukaryotic cells to isolate a protein from particular cell organelles or parts of a cell. During this process, you will look at the periplasmic fraction, which refers to the fraction that is present between the outer layer and the inner layer in the prokaryotic cells - this is the region where the bacteria stores proteins and this is where you would use the bacteria for over-expression purposes because it will give you the perfect environment for the proper folding of the protein. What are the steps for isolating the periplasmic fraction from the bacteria? This is one of the many exercises you will come to fully understand as you study this cell biology course. You will learn how to isolate cell organelles from the liver, beginning with the process of removing blood from the liver as a way of eliminating any possible contamination by the blood cells.
As you work through this free online Cell Biology Techniques in Biotechnology course content, you will gain insight into how you can use a microscope to localize a particular protein when performing different cell-based experiments and be able to perform immuno-localization studies on a mammalian cell. Finally, you will study the forward scatter channel, the side-scatter channel, phagocytosis, and phagosome and lysosome interactions.
If you are a biotechnology student or already working as a professional in Experimental Biotechnology and want to refine your cell biology skills, then kick-start your learning today with this free online course in cell biology techniques.Start Course Now
By the end of this course, you will be able to
- Discuss the steps in the preparation of a Microbiology Medium
- Describe the steps in the Isolation of the Periplasmic Fraction from bacteria
- Explain the steps in the Immuno-localization process
- Describe the basic models of Electron Microscopes
- List the materials that are required in order to perform the Malaria Parasite Life Cycle
- Discuss the four Properties of a Cell
- Describe the steps in the Scanning Electron Microscope procedure
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