An Introduction to Good Lab Practices
In this free online course, learn about the systems and principles involved in good laboratory practices.
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In this free online course, you are introduced to the world of good laboratory practices (GLP), which embodies a set of principles that provide a framework within which laboratory studies are planned, performed, monitored, recorded, reported and archived. The three levels of categorization from the Rosch approach, will also be discussed. You will be introduced to the concept of ‘buffer capacity’, a quantitative measure of the resistance to change of pH by a solution, containing a buffering agent with respect to a change in the acid or alkali concentration. You will learn that the Organization for Economic Co-operation and Development (OECD) published the principles of GLP as guidelines for any professionals that carry out experiments in a lab. You will discover why there is list of recommended appropriate personal protection equipment (PPE) to always wear in a laboratory. Demonstrations, problems, examples and assessment questions in this instructor-led video-based course, are designed to provide you with a comprehensive foundation in everything connected to GLP.
The material introduces you to the definition of GLP and the different levels of categorization. Then, the material safety data sheet (MSDS) will be discussed, which is a document provided by the manufacturer that contains information about the potential hazards of their material. You will explore the buffer solution, which is used during experiments as a means of keeping the pH at a nearly constant value in a wide variety of chemical applications. You will discover the fact that at a fixed centrifugal force and liquid viscosity, the sedimentation rate of a particle is proportional to its size and the difference between the particle density and the density of the solution. It will be clarified that the term, ‘solution’, refers to a liquid mixture in which the minor component is uniformly distributed within the major component during a laboratory experiment. You will discover that the sterilization decontamination action is appropriate to use after handling microbes that fall under biosafety level three and four because these microbes can cause serious or potentially lethal diseases through respiratory transmission in human beings.
Next, you will learn that all scientists should prepare written reports on the results of their experimental work, and this information is derived from the data recorded under the various sections of the laboratory notebook. You will gain insight into the fact that the definitional approach to categorization proposes that category memberships are determined by comparing possible exemplars to a typical member of the category whilst the exemplar approach to categorization involves determining whether an object is similar to a standard object or not. You will then discover that sedimentation refers to a physical water treatment process where high-density particles are made to settle at the bottom of a water solution. You will be taught the factors that have an influence on centrifugation, are the density of both samples and solutions, temperature, the distance of particle displacement and the rotation speed. Finally, you will study liquid handling systems, types of centrifuges, laboratory safety guidelines and the operation of laboratory instruments. This course will be of interest to biotechnology students, or those professionals in any scientific field where lab practices are of prime importance.Start Course Now
Laboratory Basics - Learning Outcomes
Good Laboratory Practices
Laboratory Safety Guidelines
Liquid Handling Systems
Operation of Laboratory Instruments
Preparation of Solutions and Buffers
Laboratory Basics - Lesson Summary
Upon successful completion of this course, you should be able to:
- Discuss the laboratory guidelines and precautions
- Describe the contents of the material safety data sheet (MSDS)
- Explain the components of liquid handling systems in the laboratory
- Discuss the uses of the water distillation unit
- Describe the different types of centrifuges
- Explain the roles of different buffer solutions
- Discuss the importance of keeping a laboratory notebook
- Describe the factors that influence centrifugation
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