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Mastering Genomics: Unveiling Insights Through RNA-Sequencing Analysis

Mastering Genomics: Unveiling Insights Through RNA-Sequencing Analysis

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  • Intermediate Level

Mastering Genomics: Unveiling Insights Through RNA-Sequencing Analysis

Elevate your practical skills in bioinformatic analysis of RNA-sequencing data during this step-by-step course

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Mastering Genomics: Unveiling Insights Through RNA-Sequencing Analysis
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Learning Outcomes

In this free course, you will learn how to:

  • Explain the details of RNA sequencing technology step by step
  • Summarize some basic concepts and descriptions about the different types of RNAs
  • Describe databases containing RNA-seq reads and download these raw data
  • Assess the overall quality of raw RNA-seq reads using FastQC tool in terms of various parameters
  • Explain the main parts and characteristics of a FASTQ file in details
  • Apply the trimming procedure to the raw RNA-seq reads using Trimmomatic tool to remove adapters
  • Generate alignment files (SAM and BAM file) after mapping the corrected RNA-seq reads to the reference genome
  • Compute the number of aligned (mapped) reads for each gene using the HTSeq tool
  • Explain the main parts and characteristics of a SAM (Sequence Alignment Map) file in details

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