Electrophoresis and Product Isolation
This electrophoresis and product isolation course covers the relevant concepts of electrophoresis in genetics.Publisher: NPTEL
CertificationView course modules
Electrophoresis and Product Isolation is a free online course from Alison that begins with an analysis of the process of characterizing the products obtained from the host cells. You will also learn about the nature of the products derived. You will discuss electrophoresis, the importance of electrophoresis, and the types of electrophoresis. You will then learn about the basic principles of staining, the types of stains, as well as the key things to look out for in a standard staining procedure. You will also study image analysis and the different variants of PAGE.
The course then explains the concept of western blotting, the determination of molecular weight, and protein-to-protein interaction. You will also analyze the process of protein sequencing as well as the sequencing of the polypeptide chain. You will study spectroscopy, the types of spectroscopy, as well as their respective functions. Next, you will learn about the small molecule chromophore and the biological chromophore.
Furthermore, you will discuss Beer-Lambert's law, the concept of UV-visible spectroscopy, and how it can be used to detect any structural changes in a molecule. Finally, you will learn about the IR spectroscopy and the phenomena on which it depends. This free online course will be of interest to students, researchers, and anyone with an interest in the field of biotechnology. So, register for this course and start your next learning journey today.
Electrophoresis - Learning Outcomes
Image Analysis and Staining
Electrophoresis - Lesson Summary
Characterization of Isolated Product
Characterization of Isolated Product - Learning Outcomes
Types of Spectroscopy
Characterization of Isolated Product - Lesson Summary
By the end of this course, you will be able to:
- Discuss the procedure and importance of product characterization
- Explain the nature of products obtained from host cells
- Explain the basic principles of electrophoresis
- Discuss the steps in a standard staining procedure
- Analyze the different variants of PAGE
- Discuss the processes involved in protein sequencing
- Analyze the steps in polypeptide chain sequencing in the correct order
- Explain the concept and significance of spectroscopy
- Discuss the two kinds of spectroscopy and their respective functions
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