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Mastering Genomics: Unveiling Insights Through RNA-Sequencing Analysis
Mastering Genomics: Unveiling Insights Through RNA-Sequencing Analysis
What You Will Learn In This Free Course
- Explain the details of RNA sequenci...
- Summarize some basic concepts and d...
- Describe databases containing RNA-s...
- Assess the overall quality of raw R...
- Explain the details of RNA sequencing technology step by step
- Summarize some basic concepts and descriptions about the different types of RNAs
- Describe databases containing RNA-seq reads and download these raw data
- Assess the overall quality of raw RNA-seq reads using FastQC tool in terms of various parameters
- Explain the main parts and characteristics of a FASTQ file in details
- Apply the trimming procedure to the raw RNA-seq reads using Trimmomatic tool to remove adapters
- Generate alignment files (SAM and BAM file) after mapping the corrected RNA-seq reads to the reference genome
- Compute the number of aligned (mapped) reads for each gene using the HTSeq tool
- Explain the main parts and characteristics of a SAM (Sequence Alignment Map) file in details
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