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Intermediate Level
Padroneggiare la genomica: svelare approfondimenti attraverso l'analisi del sequenziamento dell'RNA
422Learners already enrolled
5-6 Avg Hours
CPD Accredited
Padroneggiare la genomica: svelare approfondimenti attraverso l'analisi del sequenziamento dell'RNA
Migliora le tue abilitĂ pratiche nell'analisi bioinformatica dei dati di sequenziamento dell'RNA durante questo corso dettagliato
Nel corso attuale, praticherai un'analisi bioinformatica/computazionale completa sui dati di sequenziamento dell'RNA (RNA-Seq) senza alcuna competenza di programmazione. Verrà insegnato in modo approfondito ... Nel corso attuale, praticherai un'analisi bioinformatica/computazionale completa sui dati di sequenziamento dell'RNA (RNA-Seq) senza alcuna competenza di programmazione. Verrà insegnato in modo approfondito l'esplorazione dei database RNA-Seq e l'ottenimento di RNA-seq grezzi ed elaborati. Inoltre, è possibile acquisire buone capacità nel controllo della qualità e nel trattamento dei dati RNA-Seq grezzi. Nel frattempo, imparerai il processo di allineamento e il conteggio delle letture allineate utilizzando vari pacchetti.
COURSE PUBLISHER -
What You Will Learn In This Free Course
- Explain the details of RNA sequenci...
- Summarize some basic concepts and d...
- Describe databases containing RNA-s...
- Assess the overall quality of raw R...
- Explain the details of RNA sequencing technology step by step
- Summarize some basic concepts and descriptions about the different types of RNAs
- Describe databases containing RNA-seq reads and download these raw data
- Assess the overall quality of raw RNA-seq reads using FastQC tool in terms of various parameters
- Explain the main parts and characteristics of a FASTQ file in details
- Apply the trimming procedure to the raw RNA-seq reads using Trimmomatic tool to remove adapters
- Generate alignment files (SAM and BAM file) after mapping the corrected RNA-seq reads to the reference genome
- Compute the number of aligned (mapped) reads for each gene using the HTSeq tool
- Explain the main parts and characteristics of a SAM (Sequence Alignment Map) file in details
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