In this free online course, learn about the processes and concepts involved in Immunology Techniques.Publisher: NPTEL
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In this free online course, you are introduced to the world of Immunology Techniques. You will explore the fact that hybridoma cells contain spleen cells capable of coding an antibody, whereas myeloma cells do not produce antibodies but have the ability to grow indefinitely. The antigen that is used for immunization, which should be as pure as possible, helps reduce the generation of cross-reactive antibodies, will also be discussed. You will be introduced to the concept of cytokines and chemokines, which are extracellular signaling molecules that mediate cell to cell communication. You will learn that the competitive ELISA technique is based on the competitive binding of the primary antibody between the target antigen in a sample and the same antigen that is coated to the multi-well plate. You will discover that pixel densities on the developed X-ray film can be collected and analyzed using a transmission-mode scanner and image analysis software. Demonstrations, problems, examples and assessment questions in this instructor-led video-based course, are designed to provide you with a comprehensive foundation, which will enable you to take serious steps into this career.
The course begins by introducing you to the definition of immunology techniques and the interaction between antibodies and antigens. Then, the Direct Agglutination test that diagnoses antibodies against a large number of cellular antigens such as RBCs, bacteria, and fungi, will be discussed. You will explore Radial Immunodiffusion, which is a quantitative immunodiffusion technique used to detect the concentration of antigens by measuring the diameter of the precipitin ring formed by the interaction of the antigen and the antibody at optimal concentration. You will discover the fact that the growth and multiplication of a host organism, that is used as an expression system, requires suitable biochemical and biophysical conditions. It will be clarified that the system which protects the organism against invading pathogens is known as the immune system and that the nucleic acid synthesis is essential for the growth and multiplication of cells. You will discover that the Sandwich ELISA setup is used to measure the level of antigens in the serum. You will be taught that Agglutination refers to the process where antigens and antibodies link together and form visible aggregates.
Next, you will learn that the principle of immuno-electrophoresis is based on the movement of antigens and antibodies to opposite poles after applying an electrical current. You will gain insight into the fact that the Indirect ELISA Technique is used to measure the level of antibodies in the serum and to calculate the titre of the antibodies. The ELISA is an immunological technique that is used to measure the level of antibodies or antigens in the body fluid. You will then discover that Hemagglutination is the phenomenon which refers to the clumping of red blood cells. You will be taught that the Western Blotting Technique is used to detect the specific protein present in the crude lysate or homogenate. You will learn that the antibody acts as a central molecule governing the different types of immune responses as well as the events that make the production of antibodies possible. Finally, you will study antibody interactions, monoclonal antibody production, indirect agglutination reactions, and the Pull-Down Assay procedure. This course will be of interest to Biotechnology students, or those professionals in Experimental Biotechnology. Why wait? Start this course today and become an expert in Immunology Techniques.Start Course Now
Upon successful completion of this course, you should be able to:
- Discuss the different types of Cell-Mediated Immune Responses
- Describe the steps in the Generation of Antibodies in an organism
- Explain the interactions between an Antibody and an Antigen
- Discuss the steps in the Immunoprecipitation Assay procedure
- Explain the steps in the Sandwich ELISA Assay procedure
- List the steps in the Monoclonal Antibody Production process
- Discuss the two types of Antibody-Antigen Interactions
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