Introduction to Molecular Cloning
This free online course on Alison takes you through the concepts, processes, and significance of molecular cloning.
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Introduction to Molecular Cloning is a free online course that begins by discussing the procedure for isolating specific genes. You will learn about the various approaches which can be used to isolate genes under different scenarios. You will also be able to explain the construction of a genomic library. The screening process of the genomic library and the cDNA library will be made clear. Therefore, the different analytical tools which can be used for the process of screening will be analyzed.
The course then explains the development and history of the polymerase chain reaction as well as the different events required when setting up the reaction. You will learn about DNA replication and primers. You will be familiar with the technical aspects of the polymerase chain reactions as well as the reagents which are important for this to occur. You will gain an understanding of the process of primer designing.
Furthermore, you will learn about the enzymes involved in molecular cloning as well as their respective functions. You will be familiar with the importance of adaptor molecules and the restriction-methylase system. Finally, you will be introduced to the transforming agents known as cloning vectors and the determinants of a good vector. This free online course will be of interest to students, researchers, and anyone with an interest in the field of genetics. So, register for this course and start your next learning journey today.Start Course Now
Basics of Cloning
Basics of Cloning - Learning Outcomes
Isolation of Gene Fragment
Polymerase Chain Reaction
Technical Aspects of Polymerase Chain Reactions
Basics of Cloning - Lesson Summary
Cloning Vectors - Learning Outcomes
Molecular Tools for Cloning
Isolation of Plasmids
Cloning Vectors - Lesson Summary
By the end of this course, you will be able to:
- Discuss the different approaches to isolate a gene of interest
- Explain the protocols for the construction of the genomic library
- Analyze the stages of cell division and growth
- Discuss the discovery of the polymerase chain reaction
- List the reagents which are important for the polymerase chain reaction
- List the enzymes involved in molecular cloning and state their functions
- Discuss the importance of the restriction-methylase system
- Explain the responsibility of a cloning vector
- Discuss the criterion of a good cloning vector
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