Introduction to Chromatography System
Learn the basic concepts, types, and procedures of the chromatography system from this free online training course
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Introduction to Chromatography System is a free online training course that begins with an introduction to the procedure for the extraction of products from overexpressed cells. You will analyze the different steps involved in product processing and the various methods of cell disruption. You will also discuss the purification and isolation of the protein of interest, as well as how the separation techniques work in the case of chromatography techniques. You will also learn about the concept of resolution.
The course then explains the components of the chromatography system as well as the processes involved in the chromatography system. You will learn about the chromatogram and the different properties that are present in the protein molecules. You will also study ion-exchange chromatography, the types of ion-exchange chromatography, as well as its mode of operation. Next, you will be familiarized with the processes involved in hydrophobic interaction chromatography.
Furthermore, you will learn about gel filtration chromatography and its appropriate matrix. The course then analyzes the concept of affinitive chromatography. You will also be able to explain how to couple the receptors in order to prepare an affinity column and the generation of antibodies. This free online course will be of immense interest to students, researchers, and anyone with an interest in the processes and applications of biotechnology. So, register for this course and start your next learning journey today.
Isolation and Purification of Product
Isolation and Purification of Product - Learning Outcomes
Product Recovery from Host Cells
Basics of Chromatography
Hydrophobic Interaction Chromatography
Isolation and Purification of Product - Lesson Summary
Gel Filtration and Affinity Chromatography
Gel Filtration and Affinity Chromatography - Learning Outcomes
Gel Filtration Chromatography
Gel Filtration - Demo Session
Generation of Receptor and Antibody
Types of Affinity Chromatography
Affinity Chromatography - Demo Session
Gel Filtration and Affinity Chromatography - Lesson Summary
By the end of this course, you will be able to:
- Discuss the different steps in product processing
- Explain the physical, chemical and mechanical methods of cell disruption
- Discuss the principle of separation
- Explain the concept of resolution
- Discuss the components and processes in the chromatography system
- Discuss how the choice of matrix used in gel filtration is determined
- Analyze the procedure for detecting the different types of problems in the gel filtration column
- Explain the steps in the generation of antibody and receptor
- Discuss receptor coupling
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