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Bioreactor Design: Fed-Batch and Continuous Bioreactors

Learn how to design fed batch and continuous bioreactors and processes in biotechnology with this free online course.

Publisher: NPTEL
The bioreactor is the heart of any biochemical process. It contributes significantly to many economic activities such as agriculture, production of antibiotics, bioenergy, and environmental protection. This exciting free online course will reveal how fed-batch and continuous bioreactors are designed. Training in this interdisciplinary area would benefit many fields of human endeavour. Start learning today and be a part of the journey.
Bioreactor Design: Fed-Batch and Continuous Bioreactors
  • Duration

    3-4 Hours
  • Students

  • Accreditation






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The bioreactor is the pith of any biochemical process in which enzymes, microbial, mammalian, or plant cell systems are used for the manufacture of a wide range of useful biological products. The fermentation process can be carried out in batch, fed-batch, or continuous modes of operations. This free online course introduces you to design principles of fed-batch and continuous bioreactors to design an appropriate bioreactor for a particular bioprocess. To design a bioreactor, some objectives have to be defined. The decisions made in the design of the bioreactor might have a significant impact on the overall process. You will start by learning the definition of fed-batch cultivation. The objectives of running fed-batch operations and their advantages will be treated. Discover the mathematical model for the growth of microorganisms which is a useful tool to describe microbially cell growth, product formation and to optimize culture conditions. Uncover how the volume of biomass changes with time and the flow rate of the feed, and the product concentration changes in the reactor. Determine the concentration and total amount of biomass, substrate concentration, and product concentration quasi-steady state. Do you know what pseudo-state or steady-state means? The definitions of these terms will be covered by the course. Estimate the batch culture time and the final biomass concentration, the final mass of cells in a reactor, and how much cell biomass can be produced annually.

Next, you will gain knowledge on the basic features of an ideal continuous stirred tank reactor, and a simple schematic representation of a chemostat and turbidostat will be shown. Learn the mathematical model of continuous stirred tank reactor, how to determine the productivity of a chemostat and steady-state substrate concentration. The Monod equation will be used to describe the stimulation of growth by the concentration of nutrients, describe the substrate consumption and bacterial growth. The significance of various parameters, the relationship between growth rate and dilution rate will be explained. You will be shown the graphical representation of the relationship between cell density, nutrient concentration, and dilution rate. Compare productivity between a batch and continuous reactors, and describe the model for the plug flow reactor. A graph showing the change in the concentration of cells and substrate as a function of the residence time will be analyzed. Identify the washout point and find the dilution rate in CSTR. Learn how multiple CSTRs and PFR connected in series will operate.

Bioreactor engineering science is experiencing rapid progress. In recent years, bioreactors have received great interest. Bioreactors are the pillars of bioprocessing, supporting the production of biomass and therapeutic products. Those responsible for operating bioreactors must understand the intricacies of their functionality to ensure they can optimize their performance and maximize the bioprocess efficiency. Many different designs of bioreactors have been developed to meet different needs. In all cases, the bioreactor must provide the environmental conditions necessary for the culture. The specific demands are often conflicting and achieving optimal performance requires attaining the proper balance among the different requirements. The success of a bioprocess depends critically on the good design and operation of the bioreactor. This course will present all aspects that are relevant for an appreciation of all relevant features of bioreactors. By completing this course, you will have crucial skills that would benefit many fields of human endeavour. There is a high demand for qualified and trained engineers in this field, so enrol in this course to build up your skills, and jump-start your career. Start now!

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