Electrophoresis - Lesson Summary
Electrophoresis is an electrokinetic process which separates charged particles in a fluid using a field of electrical charge.The electrophoresis technique is not good for use in the separation and analysis of complex biological samples. The electrophoresis in the Horizontal Gel Electrophoresis system performs in a continuous way. The electrophoresis in the Vertical Gel Electrophoresis system performs in a discontinuous way with a buffer in the upper and lower tank.The electrophoresis in the Horizontal Gel System is performed in a continuous fashion with both electrodes and gel cassettes submersed within the buffer.On the native page, the sample that is prepared in the loading dye does not contain detergent or denaturing agent and as a result, the sample runs on the basis of charge.The complex biological samples are efficiently resolved in 2-D gel electrophoresis. This involves the combination of charge and molecular weight to provide much greater separation in comparison to the use of the individual property.In the Horizontal Polyacrylamide Gel Electrophoresis, the complex biological sample is resolved according to its charge and then moved to the counter charge electrode.