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    Key points covered in this module are:
    A fed-batch culture is a semi-batch operation where nutrients are fed either intermittently or continuously during the course of the operation.
    Advantages of fed-batch bioreactors include the following:

    Substrate inhibition
    High cell density
    Catabolite repression
    Crabtree effects

    The objectives of running a fed-batch operation include:

    Maximize the cell formation rate for constant cell mass yields.
    The substrate concentration maintained at a value that maximizes the specific growth rate.
    Feed rate regulated to hold the substrate concentration is constant at the desired value until the reactor is full.