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A fed-batch culture is a semi-batch operation where nutrients are fed either intermittently or continuously during the course of the operation.
Advantages of fed-batch bioreactors include the following:
Substrate inhibition
High cell density
Catabolite repression
Crabtree effects
The objectives of running a fed-batch operation include:
Maximize the cell formation rate for constant cell mass yields.
The substrate concentration maintained at a value that maximizes the specific growth rate.
Feed rate regulated to hold the substrate concentration is constant at the desired value until the reactor is full.
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