Molecular Genetics is the process of studying how a particular gene in a genome regulates a particular developmental process in the plants.
Forward Genetics is the examination of the genetic cause of an altered or abnormal phenotype introduced by a chemical mutagenesis or mutation by irradiation.
In Reverse Genetics, a particular gene is altered and the phenotype is investigated.
Forward Genetic Based Approaches
Identifying the desired developmental phenotype (Mutant)
Analyzing the inheritance pattern of the phenotype (Dominant/ Recessive)
Mapping the genetic locus associated with the phenotype (Gene mapping)
Validation of the genotype-phenotype association (Complementation)
Reverse Genetic Based Approaches
Identification and isolation of the gene for functional study (Gene of Interest)
Analyzing expression pattern of the gene (Differential, Spatial and Temporal)
Functional characterization of the gene (Gain or Loss-of-Function)
Gene interaction study (Genetic, Physical or Regulatory Interaction)
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