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Cell Biology - Lesson Summary
The growth and multiplication of a host organism that is used as an expression system require suitable biochemical and biophysical conditions.Differential Centrifugation is based on the differences in sedimentation rates of the biological properties of different sizes, shapes, and densities. homogenizer is a piece of laboratory or industrial equipment used for the homogenization of cells during the isolation of cell organelles.The repeated centrifugation of cells at progressively higher speeds will fractionate homogenates of cells into separate components.Gating refers to the section of only a certain population of cells for analysis on a plot and this provides the ability to look at parameters that are only specific to that subset. Apoptosis is a process that eliminates cells during their development, thereby maintaining a balance in the body. In an Epifluorescence Microscope, the illumination of the specimen as well as the collection of the fluorescence light is achieved by a single lens. A dichroic mirror largely reflects the light below a threshold wavelength and transmits the light above that wavelength. When conducting the Immuno-localization studies, it is critical to start with the fixation step which stops the biological activity and the relative movement of cellular components and intracellular macromolecules.Resolution is a term that refers to the minimum distance at which two distinct points of a specimen can still be seen. The resolution of a microscope is linked to the numerical aperture as well as the wavelength of light that is used to examine a specimen.In a Scanning Electron Microscope the secondary electrons that are produced by the specimen are detected in order to generate an image that contains the topological features of the specimen. The image in the Transmission Electron Microscopes is generated by the electrons that have been transmitted through a thin specimen. A typical phagocytosis of a bead is represented by the appearance of beads in the phase data and the same bead will be circled by a blue fluorescence from the filipin.In the Forward Scatter Channel, the intensity of this signal has been attributed to the cell size and the refractive index. In the Side Scatter Channel, the intensity of this signal is proportional to the amount of cytosolic structure in the cell. The electronic system converts photons to photoelectrons and also amplifies the pulse either linearly or logarithmically before digitalizing the amplified pulse.