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Module 1: Modern Techniques

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Fluorescence Resonance Energy Transfer (FRET) is widely applied to study probe-target interactions, such as protein-protein interactions, protein-DNA interactions and protein-small molecule interactions. Some popular separation and purification techniques in bio-molecular studies are High-performance liquid chromatography (HPLC) Size-exclusion chromatography Capillary electrophoresis In reverse-phased HPLC, a hydrophobic stationary phase is combined with a polar mobile phase. Mass spectrometry is a highly sensitive and modern technique for the characterization of biomolecules. High-end mass spectrometry such as MALDI is also used to study proteomics (sequence and chemistry of proteins) Molecular probes are chemically synthesized oligonucleotides used to track a target molecule. The two molecular probes used widely in research are PNA and LNA.