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Module 1: Protein Interactions

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Protein Interactions - Lesson Summary

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The Key Contents from this Module are:

Mass Spectrometry is a technique for protein Identification and analysis by the production of charged molecular species in a vacuum and their separation by electric and magnetic fields based on mass to charge (m/z) ratio.

A mass spectrometer consists of three components:

Mass Spectrometry is a technique for protein Identification and analysis by the production of charged molecular species in a vacuum and their separation by electric and magnetic fields based on mass to charge (m/z) ratio.

A mass spectrometer consists of three components:

An ion source
A mass analyzer
A detector.
Methods to study protein-protein interactions include:

Traditional approaches, e.g Immunoprecipitation, Affinity chromatography, Yeast two hybrids
High throughput approaches, e.g Protein microarrays and Label-free technologies

Interatomics is the study of interactions and their consequences between various proteins and other cellular components. The networks of all such interactions 'Interactome', aims to provide a better understanding of genome and proteome functions.

Applications of Interactomics

The functioning of uncharacterized proteins
New roles of characterized proteins
Mechanisms to regulate protein activity
Networks of protein interactions

Systems biology can be defined as a biological field which investigates the behaviour and relationship of all elements in a particular biological system.

This data can then be integrated, graphically displayed and ultimately modelled computationally. Common elements of systems biology include Networks, Modelling Computation and Dynamic properties.