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DNA Sequencing - Lesson Summary

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Maxam Gilbert DNA sequencing method:

Purely a chemical method, which means no enzyme is involved.
A destructive process, in which the target DNA strand is broken up in a controlled manner.
Requires four different chemical treatments using four vials

DNA Chemistry:
The average molar mass of a DNA monomer is approximately 330 gram per mole.
Selective radioactive labeling of DNA in the presence of RNA can be achieved with the dTTP32 monomer.
Compared to RNA, DNA is found to be more stable when treated with concentrated NaOH.
It is difficult to crystallize DNA to study its structure; however, Selenium facilitates DNA crystal formation.
Compared to an aqueous medium, a lipid medium would be favorable to a protein structure alpha-helix formation.
An amino acid that has an amide side chain can be hydrolyzed to a different amino acid.

Click Chemistry:
Developed by K. B. Sharpless
Very popular because of its use in chemistry and chemical biology
Involves metal coupling reactions
A reaction between an azide and an alkyne, in the presence of a copper catalyst forms a 5-membered triazole ring
Reactions take place at room temperature
Used in chemical ligation of DNA