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DNA Replication - Learning Outcomes

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Describe the steps in the process of DNA replication within a biological cell.
Identify the enzymes involved in the DNA replication process with their specific roles in the process
Compare the process of DNA replication within a biological cell with the PCR process
State the challenges associated with mimicking the DNA replication process of biological cells in the laboratory
Describe the steps of the polymerase chain reaction (PCR)
List the ingredients required by the PCR machine
Explain the need for taq polymerase in PCR
Describe the three stages of PCR
Discuss the steps involved in Sanger's di-deoxy method of analyzing the sequence of nucleobases of a given DNA.