Gel Filtration Chromatography - Lesson Summary
The choice of the column depends on the range of molecular weight and the pressure limit of the operating equipment.
The types of problems that one is likely to face after packing the column are:
Precipitation of protein
The two phases in Gel Filtration Chromatography is the mobile and stationary phases.
The Gel Filtration Sample is prepared in the mobile phase and it should be free of suspended particles to avoid clogging the column.
In the Gel Filtration Column, the flow of the mobile phase is used to elute the molecules from the column instead of the gradient of salt.
The molecular weight and size of a protein is related to the shape of the molecule and the relationship between its molecular weight and the radius of gyration.
The multilevel organization structures of a protein are:
The native molecular weight determination by gel filtration in conjugation with the SDS-PAGE can be used to determine the oligomeric status of the protein.
The binding of the DNA to the protein induces conformational changes, which results in the change or their size or shape.