The Key Contents from this Module are:
Plant tissue culture provides a model system to study growth and differentiation under controlled conditions.
A callus is a blob of tissue that naturally develops on a plant as a result of a wound.
Applications of plant tissue culture:
Horticulture and Forestry
Production of Phytopharmaceuticals
Products from transgenic plants
The nutrient medium for most plant tissue cultures is comprised of five groups of ingredients, namely:
1. Inorganic nutrients
2. Carbon source
4. Growth regulators
5. Organic supplements
Organogenesis is a process whereby explants, tissues or cells can be induced to form root or shoot (i.e. are forced to undergo changes which lead to the production of unipolar structures namely shoot or roo primordium) and even a whole plant.
Somatic embryogenesis is a non-sexual developmental process which produces a bipolar embryo from somatic tissue.
Somaclonal variation is defined as genetic variation observed among the progeny of plants regenerated from somatic cells cultured in vitro.
Factors affecting somaclonal variation
Source of explant
Tissue culture method
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