Chemistry - Chromatography - activity 2
Chromatography - activity 2
Shown below is a sample chromatogram for the separation of a mixture of
four saturated hydrocarbons.
(a) Which hydrocarbon contains the largest number of carbon atoms?
(b) Which hydrocarbon is most abundant in the mixture?
(c) Which hydrocarbon was least strongly attracted to the stationary
(d) What information would need to be available in order to identify
(e) What effect would increasing the length of the column have on the
(f) What effect would increasing the flow rate of the mobile phase have on
(g) A chemist, suspecting that component B is propane, adds a small amount
of propane to another sample of the mixture of the four hydrocarbons and
runs this modified sample through the chromatogram under the same
conditions. If component B is propane, how would the chromatogram of the
modified sample differ from the original chromatogram?
HPLC is particularly useful for analysing heat-sensitive materials such as
pharmaceuticals and biological materials. It is also used in food analysis
and quality control, forensic analysis and environmental testing.
Previous | Next