The Key Contents from this Module are:

The challenges for bioreactor designing for hairy root cultures are:

Shear sensitivity of the hairy root system
The requirement of a support matrix
Development of oxygen gradients within the root tissue
Mass transfer resistance due to the liquid and solid boundary areas
Resistance to flow due to the interlocked matrix because of extensive branching roots

Bioreactor Operating Strategies include:

1. An effective bioreactor operating strategy provides high productivity, a high product yield and high product content.
2. High product content makes downstream separation and purification easier. High product yield reduces the cost of substrates.
3. The operating strategy is based on the pattern of product formation and whether the product is excreted into the medium or retained in the cell.
4. To relate the pattern of product synthesis to cell growth-appropriate index for cell growth is required.