XSIQ
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Biology - Southern blotting (named after Dr Southern who invented the
process)
Southern blotting (named after Dr Southern who invented the process)
* The fragments are transferred to a nitrocellulose or nylon sheet (the
blotter)
* The DNA fragments diffuse out of the gel and onto the membrane (most
texts provide a diagram) making a profile
* The DNA fragments are treated with alkaline chemicals or heated to
separate or dissociate the paired strands (This is referred to as
denaturing the DNA).
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