XSIQ
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Biology - Southern blotting (named after Dr Southern who invented the
process)

Southern blotting (named after Dr Southern who invented the process)

* The fragments are transferred to a nitrocellulose or nylon sheet (the
blotter)

* The DNA fragments diffuse out of the gel and onto the membrane (most
texts provide a diagram) making a profile

* The DNA fragments are treated with alkaline chemicals or heated to
separate or dissociate the paired strands (This is referred to as
denaturing the DNA).

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